Probing function of O-glycosylation by an organotypic skin model

This project is dedicated to the discovery and molecular dissection of functions of protein glycosylation with a particular emphasis on O-linked protein glycosylation.

My overall aim is to identify specific regulated glycosylation events that control epithelial formation and homeostasis with broad importance for cell biology stem cell biology, epithelial homeostasis, epithelial differentiation, and cancer development in a well-defined organotypic skin model. 

Glycosylation is one of the most abundant and diverse posttranslational modifications of proteins with more than 400 glycogenes utilized in the glycosylation process. From functional proteomics studies it is becoming increasingly clear that site-specific modifications of proteins regulate and diversify functions of the proteome, and that lack of glycosyltransferases lead to a number of severe phenotypes which suggests functions in central areas including receptor biology, pro-protein processing, intracellular and extracellular-targeting, and cellular interactions. The challenge is now to identify the molecular function of glycogenes in biological systems.
Using precise genome editing techniques, which enable us to stably engineer glycosyltransferases in keratinocyte cell lines suitable for organotypic models, we will perform a discovery program of the biological function of glycosylation in epithelial formation. This will have broad importance for stem cell biology, epithelial homeostasis, epithelial differentiation, and cancer development.

The project is financed from the Danish National Research Program “Sapere Aude – Project leader”

Hans H. Wandall, MD, PhD, Associate Professor
Emil Pallesen, PhD-student 

For other activities and people associated to the laboratory please see:

Sally Dabelsteen, DDS, PhD Associate Professor