Phosphoproteomic Analysis and Organotypic Cultures for the Study of Signaling Pathways

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Standard

Phosphoproteomic Analysis and Organotypic Cultures for the Study of Signaling Pathways. / Ye, Zilu; Wandall, Hans H.; Dabelsteen, Sally.

I: Bio-protocol, Bind 14, Nr. 4, e4941, 2024.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Ye, Z, Wandall, HH & Dabelsteen, S 2024, 'Phosphoproteomic Analysis and Organotypic Cultures for the Study of Signaling Pathways', Bio-protocol, bind 14, nr. 4, e4941. https://doi.org/10.21769/BioProtoc.4941

APA

Ye, Z., Wandall, H. H., & Dabelsteen, S. (2024). Phosphoproteomic Analysis and Organotypic Cultures for the Study of Signaling Pathways. Bio-protocol, 14(4), [e4941]. https://doi.org/10.21769/BioProtoc.4941

Vancouver

Ye Z, Wandall HH, Dabelsteen S. Phosphoproteomic Analysis and Organotypic Cultures for the Study of Signaling Pathways. Bio-protocol. 2024;14(4). e4941. https://doi.org/10.21769/BioProtoc.4941

Author

Ye, Zilu ; Wandall, Hans H. ; Dabelsteen, Sally. / Phosphoproteomic Analysis and Organotypic Cultures for the Study of Signaling Pathways. I: Bio-protocol. 2024 ; Bind 14, Nr. 4.

Bibtex

@article{09545a4eb3a44becb90a6bf3ace0257e,
title = "Phosphoproteomic Analysis and Organotypic Cultures for the Study of Signaling Pathways",
abstract = "Signaling pathways are involved in key cellular functions from embryonic development to pathological conditions, with a pivotal role in tissue homeostasis and transformation. Although most signaling pathways have been intensively examined, most studies have been carried out in murine models or simple cell culture. We describe the dissection of the TGF-β signaling pathway in human tissue using CRISPR-Cas9 genetically engineered human keratinocytes (N/TERT-1) in a 3D organotypic skin model combined with quantitative proteomics and phosphoproteomics mass spectrometry. The use of human 3D organotypic cultures and genetic engineering combined with quantitative proteomics and phosphoproteomics is a powerful tool providing insight into signaling pathways in a human setting. The methods are applicable to other gene targets and 3D cell and tissue models.",
keywords = "Cell signaling, Gene editing, Keratinocytes, Organotypic model, Phosphoproteomics, Proteomics",
author = "Zilu Ye and Wandall, {Hans H.} and Sally Dabelsteen",
note = "Publisher Copyright: {\textcopyright} 2024 Bio-protocol LLC. All rights reserved.",
year = "2024",
doi = "10.21769/BioProtoc.4941",
language = "English",
volume = "14",
journal = "Bio-protocol",
issn = "2331-8325",
publisher = "bio-protocol",
number = "4",

}

RIS

TY - JOUR

T1 - Phosphoproteomic Analysis and Organotypic Cultures for the Study of Signaling Pathways

AU - Ye, Zilu

AU - Wandall, Hans H.

AU - Dabelsteen, Sally

N1 - Publisher Copyright: © 2024 Bio-protocol LLC. All rights reserved.

PY - 2024

Y1 - 2024

N2 - Signaling pathways are involved in key cellular functions from embryonic development to pathological conditions, with a pivotal role in tissue homeostasis and transformation. Although most signaling pathways have been intensively examined, most studies have been carried out in murine models or simple cell culture. We describe the dissection of the TGF-β signaling pathway in human tissue using CRISPR-Cas9 genetically engineered human keratinocytes (N/TERT-1) in a 3D organotypic skin model combined with quantitative proteomics and phosphoproteomics mass spectrometry. The use of human 3D organotypic cultures and genetic engineering combined with quantitative proteomics and phosphoproteomics is a powerful tool providing insight into signaling pathways in a human setting. The methods are applicable to other gene targets and 3D cell and tissue models.

AB - Signaling pathways are involved in key cellular functions from embryonic development to pathological conditions, with a pivotal role in tissue homeostasis and transformation. Although most signaling pathways have been intensively examined, most studies have been carried out in murine models or simple cell culture. We describe the dissection of the TGF-β signaling pathway in human tissue using CRISPR-Cas9 genetically engineered human keratinocytes (N/TERT-1) in a 3D organotypic skin model combined with quantitative proteomics and phosphoproteomics mass spectrometry. The use of human 3D organotypic cultures and genetic engineering combined with quantitative proteomics and phosphoproteomics is a powerful tool providing insight into signaling pathways in a human setting. The methods are applicable to other gene targets and 3D cell and tissue models.

KW - Cell signaling

KW - Gene editing

KW - Keratinocytes

KW - Organotypic model

KW - Phosphoproteomics

KW - Proteomics

U2 - 10.21769/BioProtoc.4941

DO - 10.21769/BioProtoc.4941

M3 - Journal article

C2 - 38410375

AN - SCOPUS:85185558977

VL - 14

JO - Bio-protocol

JF - Bio-protocol

SN - 2331-8325

IS - 4

M1 - e4941

ER -

ID: 384618638