RNA helicase-mediated regulation of snoRNP dynamics on pre-ribosomes and rRNA 2'-O-methylation

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Standard

RNA helicase-mediated regulation of snoRNP dynamics on pre-ribosomes and rRNA 2'-O-methylation. / Aquino, Gerald Ryan R.; Krogh, Nicolai; Hackert, Philipp; Martin, Roman; Gallesio, Jimena Davila; van Nues, Robert W.; Schneider, Claudia; Watkins, Nicholas J.; Nielsen, Henrik; Bohnsack, Katherine E.; Bohnsack, Markus T.

I: Nucleic Acids Research, Bind 49, Nr. 7, 2021, s. 4066-4084.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Aquino, GRR, Krogh, N, Hackert, P, Martin, R, Gallesio, JD, van Nues, RW, Schneider, C, Watkins, NJ, Nielsen, H, Bohnsack, KE & Bohnsack, MT 2021, 'RNA helicase-mediated regulation of snoRNP dynamics on pre-ribosomes and rRNA 2'-O-methylation', Nucleic Acids Research, bind 49, nr. 7, s. 4066-4084. https://doi.org/10.1093/nar/gkab159

APA

Aquino, G. R. R., Krogh, N., Hackert, P., Martin, R., Gallesio, J. D., van Nues, R. W., Schneider, C., Watkins, N. J., Nielsen, H., Bohnsack, K. E., & Bohnsack, M. T. (2021). RNA helicase-mediated regulation of snoRNP dynamics on pre-ribosomes and rRNA 2'-O-methylation. Nucleic Acids Research, 49(7), 4066-4084. https://doi.org/10.1093/nar/gkab159

Vancouver

Aquino GRR, Krogh N, Hackert P, Martin R, Gallesio JD, van Nues RW o.a. RNA helicase-mediated regulation of snoRNP dynamics on pre-ribosomes and rRNA 2'-O-methylation. Nucleic Acids Research. 2021;49(7):4066-4084. https://doi.org/10.1093/nar/gkab159

Author

Aquino, Gerald Ryan R. ; Krogh, Nicolai ; Hackert, Philipp ; Martin, Roman ; Gallesio, Jimena Davila ; van Nues, Robert W. ; Schneider, Claudia ; Watkins, Nicholas J. ; Nielsen, Henrik ; Bohnsack, Katherine E. ; Bohnsack, Markus T. / RNA helicase-mediated regulation of snoRNP dynamics on pre-ribosomes and rRNA 2'-O-methylation. I: Nucleic Acids Research. 2021 ; Bind 49, Nr. 7. s. 4066-4084.

Bibtex

@article{f05dbd9f37e644969e9d9fa8b2b2a9fa,
title = "RNA helicase-mediated regulation of snoRNP dynamics on pre-ribosomes and rRNA 2'-O-methylation",
abstract = "RNA helicases play important roles in diverse aspects of RNA metabolism through their functions in remodelling ribonucleoprotein complexes (RNPs), such as pre-ribosomes. Here, we show that the DEAD box helicase Dbp3 is required for efficient processing of the U18 and U24 intron-encoded snoRNAs and 2'-O-methylation of various sites within the 25S ribosomal RNA (rRNA) sequence. Furthermore, numerous box C/D snoRNPs accumulate on pre-ribosomes in the absence of Dbp3. Many snoRNAs guiding Dbp3-dependent rRNA modifications have overlapping pre-rRNA basepairing sites and therefore form mutually exclusive interactions with pre-ribosomes. Analysis of the distribution of these snoRNAs between pre-ribosome-associated and 'free' pools demonstrated that many are almost exclusively associated with pre-ribosomal complexes. Our data suggest that retention of such snoRNPs on pre-ribosomes when Dbp3 is lacking may impede rRNA 2'-O-methylation by reducing the recycling efficiency of snoRNPs and by inhibiting snoRNP access to proximal target sites. The observation of substoichiometric rRNA modification at adjacent sites suggests that the snoRNPs guiding such modifications likely interact stochastically rather than hierarchically with their pre-rRNA target sites. Together, our data provide new insights into the dynamics of snoRNPs on pre-ribosomal complexes and the remodelling events occurring during the early stages of ribosome assembly.",
author = "Aquino, {Gerald Ryan R.} and Nicolai Krogh and Philipp Hackert and Roman Martin and Gallesio, {Jimena Davila} and {van Nues}, {Robert W.} and Claudia Schneider and Watkins, {Nicholas J.} and Henrik Nielsen and Bohnsack, {Katherine E.} and Bohnsack, {Markus T.}",
note = "Publisher Copyright: {\textcopyright} The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.",
year = "2021",
doi = "10.1093/nar/gkab159",
language = "English",
volume = "49",
pages = "4066--4084",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "7",

}

RIS

TY - JOUR

T1 - RNA helicase-mediated regulation of snoRNP dynamics on pre-ribosomes and rRNA 2'-O-methylation

AU - Aquino, Gerald Ryan R.

AU - Krogh, Nicolai

AU - Hackert, Philipp

AU - Martin, Roman

AU - Gallesio, Jimena Davila

AU - van Nues, Robert W.

AU - Schneider, Claudia

AU - Watkins, Nicholas J.

AU - Nielsen, Henrik

AU - Bohnsack, Katherine E.

AU - Bohnsack, Markus T.

N1 - Publisher Copyright: © The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

PY - 2021

Y1 - 2021

N2 - RNA helicases play important roles in diverse aspects of RNA metabolism through their functions in remodelling ribonucleoprotein complexes (RNPs), such as pre-ribosomes. Here, we show that the DEAD box helicase Dbp3 is required for efficient processing of the U18 and U24 intron-encoded snoRNAs and 2'-O-methylation of various sites within the 25S ribosomal RNA (rRNA) sequence. Furthermore, numerous box C/D snoRNPs accumulate on pre-ribosomes in the absence of Dbp3. Many snoRNAs guiding Dbp3-dependent rRNA modifications have overlapping pre-rRNA basepairing sites and therefore form mutually exclusive interactions with pre-ribosomes. Analysis of the distribution of these snoRNAs between pre-ribosome-associated and 'free' pools demonstrated that many are almost exclusively associated with pre-ribosomal complexes. Our data suggest that retention of such snoRNPs on pre-ribosomes when Dbp3 is lacking may impede rRNA 2'-O-methylation by reducing the recycling efficiency of snoRNPs and by inhibiting snoRNP access to proximal target sites. The observation of substoichiometric rRNA modification at adjacent sites suggests that the snoRNPs guiding such modifications likely interact stochastically rather than hierarchically with their pre-rRNA target sites. Together, our data provide new insights into the dynamics of snoRNPs on pre-ribosomal complexes and the remodelling events occurring during the early stages of ribosome assembly.

AB - RNA helicases play important roles in diverse aspects of RNA metabolism through their functions in remodelling ribonucleoprotein complexes (RNPs), such as pre-ribosomes. Here, we show that the DEAD box helicase Dbp3 is required for efficient processing of the U18 and U24 intron-encoded snoRNAs and 2'-O-methylation of various sites within the 25S ribosomal RNA (rRNA) sequence. Furthermore, numerous box C/D snoRNPs accumulate on pre-ribosomes in the absence of Dbp3. Many snoRNAs guiding Dbp3-dependent rRNA modifications have overlapping pre-rRNA basepairing sites and therefore form mutually exclusive interactions with pre-ribosomes. Analysis of the distribution of these snoRNAs between pre-ribosome-associated and 'free' pools demonstrated that many are almost exclusively associated with pre-ribosomal complexes. Our data suggest that retention of such snoRNPs on pre-ribosomes when Dbp3 is lacking may impede rRNA 2'-O-methylation by reducing the recycling efficiency of snoRNPs and by inhibiting snoRNP access to proximal target sites. The observation of substoichiometric rRNA modification at adjacent sites suggests that the snoRNPs guiding such modifications likely interact stochastically rather than hierarchically with their pre-rRNA target sites. Together, our data provide new insights into the dynamics of snoRNPs on pre-ribosomal complexes and the remodelling events occurring during the early stages of ribosome assembly.

U2 - 10.1093/nar/gkab159

DO - 10.1093/nar/gkab159

M3 - Journal article

C2 - 33721027

AN - SCOPUS:85105688599

VL - 49

SP - 4066

EP - 4084

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 7

ER -

ID: 269492553