HC Bisgaard Group – University of Copenhagen

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Department of Cellular and Molecular Medicine > Research Groups > HC Bisgaard Group

HC Bisgaard Group

Morphogenesis and Differentiation Program

Research areas

  • Characterization of cellular and molecular mechanisms involved in foetal and adult programming of the mammalian liver with specific emphasis on differentiation of stem/progenitor cells into hepatocytes and cholangiocytes.

  • In vitro programming and differentiation of human hepatic progenitor cells (hHB/HepaRG), human embryonic stem cells (hESC), and induced pluripotent stem cells (hiPSC) along hepatic cell lineages with special emphasis on nutritional effects of the microenvironment

  • Identification of novel molecular mechanisms involved in repair and regeneration of an injured liver using experimental animal models in mouse and rat.

Current research areas

  • Investigations aimed at elucidating a possible role of hexose (glucose versus fructose) induced changes in mitochondrial phenotype during programming of liver cell differentiation
  • Generation of an in vitro system for studying hepatic differentiation and hepatic associated diseases using induced pluripotent stem cells and gene editing

Collaborators

Bjørn Quistorff, Professor, Department of Biomedical Sciences, SUND, University of Copenhagen, Denmark
Lene Juel Rasmussen, Professor, Center for Healthy Aging, Department of Cellular and Molecular Medicine, SUND, University of Copenhagen, Denmark
Lisbeth Birk Møller, Senior Research Sceintist, Medical Genetics Laboratory, The Kennedy Center, University of Copenhagen, Denmark
Bjørn Holst, R&D Manager Disease Models, and Mikkel Aabech (post doc), Bioneer A/S, Hørsholm, Denmark
Peter Nagy, Professor, Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary

Research based teaching

Hanne Cathrine Bisgaard has been involved in pre- and post-graduate teaching in Chemistry, Cell Biology, Biochemistry, and Endocrinology at SUND/KU and Cell Biology and Biotechnology at Roskilde University. Currently, our group is mainly involved in teaching medical and odontology undergraduate students the chemistry of cellular components in basal human biology at SUND/KU.
In addition, we supervise 2-4 human biology and medical master students in our laboratory per year.
Hanne Cathrine Bisgaard is the administrator of the 1. Semester theoretical course and the integrated obligatory laboratory experimental course entitled “The Chemical Components of the Cell” (275 medical students twice per year/98 odontology students once per year at SUND/KU). 
Furthermore, Hanne Cathrine Bisgaard has supervised a number of graduate students and is an active evaluator of Ph.D.-dissertations at both national and international universities.

Recent publications

  • Hopkinson BM, Desler C, Kalisz M, Vestentoft PS, Rasmussen LJ, Bisgaard HC (2017). Bioenergetic changes during differentiation of human embryonic stem cells along the hepatic lineage. Oxidative Medicine and Cellular Longevity. 2017:5080128.

  • Vestentoft PS, Jelnes P, Andersen JB, Tran TAT, Jørgensen T, Rasmussen M, Lange JB, Grøvdal LM, Jensen CH, Vogel LK, Thorgeirsson SS, Bisgaard HC (2013). Molecular constituents of the remodeling extracellular matrix in injured rat liver with a hepatic progenitor cell response. Fibrogenesis & Tissue Repair 6:21.

  • Jakobsen JS, Waage J, Rapin N, Bisgaard HC, Larsen FS, Porse BT (2013). Temporal mapping of C/EBPα and –β binding during liver regeneration reveals dynamic occupancy and specific regulatory codes for homeostatic and cell cycle gene batteries. Genome Res.  23(4):592-603.

  • Eefsen M, Vainer B, Bisgaard HC, Larsen FS (2012). Cerebral Expression of NKCC1 in Rats with Acute and Chronic Hyperammonemia. J Liver: Dis. Transplant. 1:2

  • Kalisz M, Winzi M, Bisgaard HC, Serup P (2012). Opposing actions of EVX1 and GOOSECOID mediate embryonic stem cell fate to TGFβ. Dev Biol. 362(1):94-103.

  • Vestentoft PS, Jelnes P, Hopkinson BH, Vainer B, Møllgård K, Quistorff Q, Bisgaard HC (2011). Three-dimensional reconstructions of intrahepatic bile duct tubulogenesis in human liver. BMC Developmental Biology 11:56.

  • Bornholdt J, Friis S, Godiksen S, Poulsen SS, Santoni-Rugiu E, Bisgaard HC, Lothe IM, Ikdahl T, Tveit KM, Johnson E, Kure EH, Vogel LK. (2011).The level of Claudin-7 is reduced as an early event in colorectal carcinogenesis. BMC Cancer 11:65.

Selected publications

  • Hopkinson BM, Desler C, Kalisz M, Vestentoft PS, Rasmussen LJ, Bisgaard HC (2017). Bioenergetic changes during differentiation of human embryonic stem cells along the hepatic lineage. Oxidative Medicine and Cellular Longevity. 2017:5080128.

  • Vestentoft PS, Jelnes P, Andersen JB, Tran TAT, Jørgensen T, Rasmussen M, Lange JB, Grøvdal LM, Jensen CH, Vogel LK, Thorgeirsson SS, Bisgaard HC (2013). Molecular constituents of the remodeling extracellular matrix in injured rat liver with a hepatic progenitor cell response. Fibrogenesis & Tissue Repair 6:21.

  • Kalisz M, Winzi M, Bisgaard HC, Serup P (2012). Opposing actions of EVX1 a
    nd GOOSECOID mediate embryonic stem cell fate to TGFβ. Dev Biol. 362(1):94-103.

  • Vestentoft PS, Jelnes P, Hopkinson BH, Vainer B, Møllgård K, Quistorff Q, Bisgaard HC (2011).Three-dimensional reconstructions of intrahepatic bile duct tubulogenesis in human liver. BMC Developmental Biology 11:56.

  • Dezső K, Halász J, Bisgaard HC, Paku S, Schaff Z, Nagy P (2008). Delta-like protein (DLK) is a novel immunohistochemical marker for human hepatoblastomas. Virchows Archiv. 452:443-448.

  • Dezső K, Jelnes P, Laszlo V, Baghy K, Paku S, Bisgaard HC*, Nagy P* (2007). Thy-1 is expressed in hepatic myofibroblasts and not oval cells in stem cell-mediated liver regeneration. Am. J.  Pathol. 171:1529-37. * these authors contributed equally as senior investigators in this study.

  • Jelnes P, Santoni-Rugiu E, Rasmussen M, Friis SL, Nielsen JH, Tygstrup N, Bisgaard HC (2007). Remarkable phenotypic heterogeneity displayed by oval cells in rat and mouse models of stem cell-mediated liver regeneration. Hepatology 45:1462-1470

  • Jensen CH, Jauho ES, Santoni-Rugiu E, Holmskov U, Teisner B, Tygstrup N, Bisgaard HC (2004) Transit-amplifying progenitor (oval) cells and their hepatocytic progeny in the regenerating rat liver are characterized by a distinctive expression of delta-like protein/preadipocyte factor 1/fetal antigen 1 (2004). Am. J. Pathol. 164:1347-1359.

  • Bisgaard HC, Holmskov U, Santoni-Rugiu E, Nagy P, Nielsen O, Ott P, Hage E, Dalhoff K, Rasmussen LJ, and Tygstrup N (2002). Heterogeneity of ductular reactions in adult rat and human liver revealed by novel expression of Deleted in Malignant Brain Tumor 1. Am.J. Pathol. 161 :1187-1198.

  • Jäger AC, Rasmussen M, Bisgaard HC, Singh KK, Nielsen FC, Rasmussen LJ. (2001). HNPCC mutations in the human DNA mismatch repair gene hMLH1 influence assembly of hMutLalpha and hMLH1-hEXO1 complexes. Oncogene 20:3590-5.