López-Contreras Group – University of Copenhagen

López-Contreras Group

Molecular Aging Program
Center for Chromosome Stability
Center for Healthy Aging

Research Interests

DNA damage and DNA repair, Replication Stress, Mouse models of cancer and ageing.

Current Research

Exploring the impact of Replication Stress on Ageing and Cancer This project is financed from the Danish National Research Program “Sapere Aude – DFF Starting Grant

Identification of novel targets for the BRCA1 ubiquitin ligase activity. This project is funded by a KBVU Project from the Danish Cancer Society.

Chromosomal Common Fragile Sites: Unravelling their biological functions and the basis of their instability This project is funded by the European Research Council (ERC-2015-StG)

Participants: Andres J Lopez-Contreras, group leader Alexandra Avram, lab technician Mauro Sbroggió, postdoc  Stephanie Munk, postdoc Eliene Albers, PhD student David Pladevall Morera, PhD student

If you are interested in joining our lab, we want to hear about you and your ideas! Please, send an e-mail to ajlopez@sund.ku.dk including your CV and a motivation letter.

Collaborators

Dr. Oscar Fernandez-Capetillo, Spanish National Cancer Research Center (CNIO), Madrid, Spain
Prof. Ian D. Hickson, Department of Cellular and Molecular Medicine, University of Copenhagen, Denmark
Prof. Rafael Peñafiel-Garcia, University of Murcia, Spain
Dr. Javier Muñoz, Spanish National Cancer Research Center (CNIO), Madrid, Spain
Dr. Andre Nussenzweig, National Cancer Institute, National Institutes of Health, Bethesda, USA
Dr. Jeremy A. Daniel, Center for Protein Research, University of Copenhagen, Denmark.
Jesper V. Olsen, CPR, Copenhagen, Denmark (mass spectrometry for quantitative proteomics)
Lene J. Rasmussen, CEHA, Copenhagen, Denmark (genomic instability and mitochondrial function)

Recent publications:           

  • Albers E, Sbroggiò M, Pladevall-Morera D, Bizard AH, Avram A, Gonzalez P, Martin-Gonzalez J, Hickson ID, Lopez-Contreras AJ. Loss of PICH Results in Chromosomal Instability, p53 Activation, and Embryonic Lethality.
    Cell Rep (2018)

  • Lambertos A, Ramos-Molina B, Cerezo D, López-Contreras AJ, Peñafiel R. The mouse Gm853 gene encodes a novel enzyme: Leucine decarboxylase.
    Biochim Biophys Acta Gen Subj. (2018)

  • Munk S, Sigurðsson JO, Xiao Z, Batth TS, Franciosa G, von Stechow L, Lopez-Contreras AJ, Vertegaal ACO, Olsen JV. Proteomics Reveals Global Regulation of Protein SUMOylation by ATM and ATR Kinases during Replication Stress.
    Cell Rep (2017)

  • Albers E, Sbroggiò M, Martin-Gonzalez J, Avram A, Munk S, Lopez-Contreras AJ. A simple DNA recombination screening method by RT-PCR as an alternative to Southern blot.
    Transgenic Res. (2017)

  • Herrtwich L, Nanda I, Evangelou K, Nikolova T, Horn V, Sagar, …, Lopez-Contreras AJ, Grün D, Gorgoulis V, Diefenbach A, Henneke P, Triantafyllopoulou A. DNA Damage Signaling Instructs Polyploid Macrophage Fate in Granulomas. 
    Cell (2016)

Selected publications:

  • Lopez-Contreras AJ*, Specks J, Barlow JH, Ambrogio C, Desler C, Vikingsson S, Rodrigo-Perez S, Green H, Rasmussen LJ, Murga M, Nussenzweig A, Fernandez-Capetillo O*. Increased Rrm2 gene dosage reduces fragile site breakage and prolongs survival of ATR mutant mice. Genes Dev (2015) Apr 1. *Co-corresponding authors.

  • Specks J, Nieto-Soler M, Lopez-Contreras AJ*, Fernandez-Capetillo O*. Modeling the study of DNA damage responses in mice. Methods Mol Biol (2015). *Co-corresponding authors.

  • Schulze J*, Lopez-Contreras AJ*, Uluçkan O, Graña-Castro O, Fernandez-Capetillo O, Wagner EF. Fos-dependent induction of Chk1 protects osteoblasts from replication stress. Cell Cycle, 13: 1980-1986 (2014)   * co-first authors

  • Lopez-Contreras AJ, Ruppen I, Nieto-Soler M, Murga M, Rodriguez-Acebes S, Remeseiro S, Rodrigo-Perez S, Rojas A, Mendez J, Muñoz J, Fernandez-Capetillo O. A proteomic characterization of factors enriched on nascent DNA molecules. Cell Reports, 3: 1105-1116 (2013)

  • Marques-Torrejon MA, Porlan E, Banito A, Gomez-Ibarlucea E, Lopez-Contreras AJ, Fernandez-Capetillo O, Vidal A, Gil J, Torres J, Fariñas I. Cyclin-dependent kinase inhibitor p21 controls adult neural stem cell expansion by regulating Sox2 gene expression. Cell Stem Cell, 12: 88-100 (2013)

  • Bunting SF, Callen E, Kozak ML, Kim JM, Wong N, Lopez-Contreras AJ, Ludwig T, Baer R, Faryabi RB, Malhowski A, Chen HT, Fernandez-Capetillo O, D'Andrea A, Nussenzweig A. BRCA1 functions independently of homologous recombination in DNA interstrand crosslink repair. Mol Cell, 46: 125-135 (2012)

  • Lopez-Contreras AJ, Gutierrez-Martinez P, Specks J, Rodrigo-Perez S, Fernandez-Capetillo O. An extra allele of Chk1 limits oncogene-induced replicative stress and promotes transformation. J Exp Med, 209:455-461 (2012)

  • Murga M, Campaner S, Lopez-Contreras AJ, Toledo LI, Soria R, Montaña MF, D'Artista L, Schleker T, Guerra C, Garcia E, Barbacid M, Hidalgo M, Amati B, Fernandez-Capetillo O. Exploiting oncogene-induced replicative stress for the selective killing of Myc-driven tumors. Nat Struct Mol Biol, 18: 1331-1335 (2011)

  • Lopez-Contreras AJ, Fernandez-Capetillo O. The ATR barrier to replication-born DNA damage. DNA Repair, 9: 1249-55 (2010)

  • Santos MA, Huen MS, Lopez-Contreras AJ, Klein IA, Jankovic M, Chen H, Wong N, Rodriguez-Barbancho JL, Fernandez-Capetillo O, Nuzzenzweig M, Chen J, Nussenzweig A. RNF8-dependent histone ubiquitinylation contributes to class-switch recombination and meiosis. J Exp Med, 207: 973-81 (2010)